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Abstract

 BACKGROUND:Human papilloma virus has been linked to many types of cervical lesions, ranging from the relativelyinnocuous lesions to fatal invasive squamous cell carcinoma. There is growing evidence of Humanpapillomavirus being a relevant factor in other anogenital cancers (anus, vulva, vagina and penis) aswell as head and neck cancers.OBJECTIVE:To assess the feasibility of immunohistochemical staining paraffin sections for the presence ofHuman papilloma virus with monoclonal antibodies (clone K1H8, IgG) raised against the major coatfusion capsid proteins and to detect the frequency of human papilloma virus immunoexpression inbenign, preneoplastic and neoplastic cervical lesions in patients living in Erbil city ( North of Iraq).MATERIALS AND METHODS:A total of 75 paraffin blocks samples of cervical tissue were retrieved retrospectively from thePathology Department of Maternity Teaching Hospital and some private laboratories in Erbil city,during a period spanning from September 2013 to June 2014. They were categorized as: Benigncervicitis (10) samples, cervical intraepithelial neoplasia, CIN I (33) samples, CIN II (10) samples,CIN III (13) samples, cervical squamous cell carcinoma (6) samples and three samples with cervicaladenocarcinoma Immunohistochemistry was performed on those samples using the avidin -biotinperoxidase complex in which primarily monoclonal anti Human papilloma virus antibodies was used.RESULTS:None of the 10 samples of benign cervicitis were positive for Human papillomavirus protein while 21out of 33 (63.6%) samples of CINI, 9 out of 10 (90%) samples of CIN II and 9 out of 13 (69.2%)samples of CIN III were positive for HPV. Also Human papillomavirus positivity observed in all sixsamples of squamous cell carcinoma (100%), mostly in sheets of less mature squamous cells and in 1out of 3 (33.3%) samples of adenocarcinoma , mostly focal and in single cell.CONCLUSION:The immunohistochemical staining technique revealed a significant detection of HPV protein incervical intraepithelial neoplasia and cervical carcinoma.

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