Abstract
ABSTRACT: BACKGROUND: HLA-B27 can effect clinical presentation and course of ankylosing spondylitis. Different detection techniques of HLA-B27 are available with variable sensitivities and specificities. OBJECTIVE: To compare serologic and molecular diagnostic techniques of detecting HLA-B27 status and to correlate it with some clinical variables among ankylosing spondylitis patients. PATIENTS AND METHODS: A cross-sectional study was conducted on 83 Iraqi patients with ankylosing spondylitis. Clinical and laboratory evaluations were reported. HLA-B27 status was determined in all patients by real-time PCR using HLA-B27 RealFast™ kit; ELISA method was used as well to detect soluble serum HLA-B27 antigens using Human Leukocyte Antigen® kit. RESULTS: The mean age of patients ± SD was (38.4±9.8) years. Male to female ratio was 9:1. Disease onset occurred
Recommended Citation
Al-Osami, Mohammed H; Al-Musawi, Bassam MS; and Abdulhadi, Mayada MA
(2018)
"Molecular and Serologic Detection of HLA-B27 among Ankylosing Spondylitis Patients with Some Clinical Correlations,"
Iraqi Postgraduate Medical Journal: Vol. 17:
Iss.
3, Article 9.
Available at:
https://www.ipmj.org/journal/vol17/iss3/9