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Abstract

BACKGROUND: Gliomas are the most common primary malignant brain tumors in adults. They can occur anywhere in the central nervous system but primarily occur in the brain and arise in the glial tissue. O6-alkylguanine DNA alkyl transferase is a protein (DNA repair molecule) encoded by the O6-methylguanine DNA methyltransferase (MGMT) gene in human, and are able to remove alkyl adducts from the O6 position of guanine, and the O4 position of thymine, restoring these DNA bases and preventing temozolomide induced cell death. OBJECTIVE: To assess the immunohistochemical expression of MGMT in human glioma. METHOD: This retro and prospective study included 56 tissue paraffin blocks of intracranial gliomas assigned as cases group, and 28 tissue paraffin blocks of normal brain tissue as control group. From each block, two sections were taken; one was stained with the routine hematoxylin and eosin stain, and the other was stained immunohistochemically for marker MGMT. RESULTS: MGMT showed a highly significant difference in its expression between the control group and the disease group (p value<0.001). MGMT showed a highly significant relation between its expression and the age of patients with glioma (p value< 0.001). MGMT expression showed no significant correlation with other clinicopathological parameters like the histological types and gender (p value CONCLUSION: MGMT revealed a highly significant difference inits expression in brain tumors (gliomas) compared to control groupwhich mayreflects the need in proliferating cells for greater capacity to repair O6-alkylguanineadducts before replication. Besides there was a significant correlation between age and MGMT expression which may reflect processes associated with the physical and functional maturation of the CNS during life.    

DOI

10.52573/ipmj.2020.168624

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