The Location of Aerobactin Determinants of Uropathogenic E. coli in Association with Hemolysin Production Antibiotic Resistance and Patient Characteristics.

Authors

• Ibtesam Ghadban Auda

Abstract

ABSTRACT: BACKGROUND: Aerobactin, Hemolysin and the resistance to some antibiotics axe important Features of Uropathogenic E. coli (UPEC). The characteristics of patients from which we isolate the UPEC have associate with these Features and their determinants location. AIM: We determined the phenotypic expression and gene location of Aerobactin and phenotypic expression of Hemolysin among 60 UPEC isolates. We correlated the presence of Aerobactin system with antibacterial agents resistance. The expression of Hemolysin and the characteristics of patients. METHODS: Two methods of plasmid curin, sodium dodecyl sulfate and elevated temperature plasmid curing, are used then plasmid extraction and agarose gel electrophoresis are performed to determine the location of Aerobactin determinants and the size of Aerobactin plasmids, as well as the location of the determinants of some antibiotics resistance which were ampicillin (Am), Tetracycline (Tc), gentamicin (GM), Chloramphenicol (C) and co- trimazole (Co). RESULTS: Aerobactin and hemolysin expression among UPEC isolates were 93.3% and 35% respectively. The isolates of non compromised patients produce statistically higher rate of expressed hemolysin (90.5% , p

Recommended Citation

Auda, Ibtesam Ghadban (2006) "The Location of Aerobactin Determinants of Uropathogenic E. coli in Association with Hemolysin Production Antibiotic Resistance and Patient Characteristics.," Iraqi Postgraduate Medical Journal: Vol. 5: Iss. 2, Article 17.
Available at: https://www.ipmj.org/journal/vol5/iss2/17